Guide RNA - PDFSEARCH.IO - Document Search Engine

Guide RNA
Results: 70

#	Item
21	Design of gRNA and construction of gRNA expression vectors Originally developed by Hodaka Fujii on February 19, 2014 Modified by Hodaka Fujii on January 10, 2015 Construction of gRNA expression vectors is done basically Add to Reading List Source URL: www.addgene.org Language: English - Date: 2015-01-22 10:39:59 Guide RNA Vector Retrovirus Nucleosome Promoter Restriction enzyme Viral vector Gene expression Expression vector Biology Molecular biology Biochemistry
22	gRNA Synthesis Protocol STEP 1: Find all 23bp genomic sites of the form 5’-N20NGG-3’ near your intended target site (ideally ±50bp). These may reside on the + or – strand. PAM 5’!NNNNN NNNNN NNNNN NNNNN NGG!3’ Add to Reading List Source URL: www.addgene.org Language: English - Date: 2014-11-05 23:32:09 Chemistry Guide RNA Polymerase chain reaction Oligonucleotide Molecular cloning Vector DNA microarray Gene expression Biology Molecular biology Biochemistry
23	CRISPR plasmids from Chen and Wente labs We have synthesized a Cas9 coding sequence with nuclear localization signals (nls) at both its amino and carboxyl termini and codons optimized for zebrafish expression (nlszCas9-n Add to Reading List Source URL: www.addgene.org Language: English - Date: 2014-11-05 23:32:00 Genetics Molecular genetics DNA ligase DNA replication Guide RNA Nuclear localization sequence Transformation DNA Ligation Biology Molecular biology RNA
24	Choice of gRNAs for the Cas9 Orthologs S. pyogenes Cas9 (SP): Find all 23bp genomic sites of the form 5’-NNNNNNNNNNNNNNNNNNNNNGG-3’ near your intended target site (ideally ±50bp). These may reside on the + or – st Add to Reading List Source URL: www.addgene.org Language: English - Date: 2014-11-05 23:31:59 Molecular biology Genetics DNA Gene expression RNA Biology Guide RNA
25	OligArch: A software tool to allow artificially expanded genetic information systems (AEGIS) to guide the autonomous self-assembly of long DNA constructs from multiple DNA single strands Kevin M. Bradley1,2 and Steven A. Add to Reading List Source URL: ffame.org Language: English - Date: 2015-04-24 17:42:13 DNA Nucleic acids Molecular biology Helices Oligonucleotide Primer Nucleic acid sequence Polymerase chain reaction RNA Biology Chemistry Genetics
26	CRISPR plasmids from Chen and Wente labs We have synthesized a Cas9 coding sequence with nuclear localization signals (nls) at both its amino and carboxyl termini and codons optimized for zebrafish expression (nlszCas9-n Add to Reading List Source URL: www.addgene.org Language: English - Date: 2014-11-05 23:32:00 Genetics Molecular genetics DNA ligase DNA replication Guide RNA Nuclear localization sequence Transformation DNA Ligation Biology Molecular biology RNA
27	Choice of gRNAs for the Cas9 Orthologs S. pyogenes Cas9 (SP): Find all 23bp genomic sites of the form 5’-NNNNNNNNNNNNNNNNNNNNNGG-3’ near your intended target site (ideally ±50bp). These may reside on the + or – st Add to Reading List Source URL: www.addgene.org Language: English - Date: 2014-11-05 23:31:59 Molecular biology Genetics DNA Gene expression RNA Biology Guide RNA
28	gRNA Synthesis Protocol STEP 1: Find all 23bp genomic sites of the form 5’-N20NGG-3’ near your intended target site (ideally ±50bp). These may reside on the + or – strand. PAM 5’!NNNNN NNNNN NNNNN NNNNN NGG!3’ Add to Reading List Source URL: www.addgene.org Language: English - Date: 2014-11-05 23:32:02 Chemistry Guide RNA Polymerase chain reaction Oligonucleotide Molecular cloning Vector DNA microarray Gene expression Biology Molecular biology Biochemistry
29	Identification of high-efficiency 3’GG gRNA motifs in indexed FASTA files with ngg2 1 PrePrints Add to Reading List Source URL: peerj.com Language: English - Date: 2015-04-08 18:10:41 Genomics Genetic mapping Guide RNA RNA Molecular biology Genome engineering CRISPR Human genome Genome Genetics Biology Bioinformatics
30	Report fluorescent. Homologous recombination (HR) using an appropriate repair donor can restore the normal GFP sequence, enabling us to quantify the resulting GFP+ cells by flow activated cell sorting (FACS). To test the Add to Reading List Source URL: arep.med.harvard.edu Language: English - Date: 2013-01-04 11:15:13 RNA Molecular biology Genetic mapping CRISPR Genome engineering Zinc finger nuclease Guide RNA Non-coding RNA DNA Biology Genetics Genomics